A new research study reveals how a common type of epigenetic modification can be transmitted via sperm not only from parents to offspring, but to the next generation (“grandoffspring”) as well.
Changing the epigenetic marks on chromosomes results in altered gene expression in offspring and in grandoffspring, demonstrating ‘transgenerational epigenetic inheritance.’
Without changing the genetic code in the
In a study of epigenetic inheritance, researchers created embryos of the worm C. elegans that inherited egg chromosomes properly packaged with the epigenetic mark H3K27me3 and sperm chromosomes lacking the mark. The one-cell embryo on the left inherited the pink chromosomes from the egg and the green chromosomes from the sperm, the colors showing the presence or absence of H3K27me3. The two-cell embryo on the right shows the egg and sperm chromosomes united in each nucleus. Credit: Photo by Laura Gaydos
In the recent work, this histone mark was selectively stripped from the chromosomes of C. elegans sperm, which were then used to fertilize eggs with fully marked chromosomes. In the resulting offspring, the scientists observed abnormal gene expression patterns, with genes on the paternal chromosomes (inherited from the sperm) turned on or “upregulated” in the absence of the repressive epigenetic mark.
This resulted in tissues turning on genes they would not normally express. For instance, germline tissue (which produces eggs and sperm) turned on genes normally expressed in neurons.
“In all the tissues we analyzed, genes were aberrantly expressed, but different genes were turned up in different tissues, demonstrating that the tissue context determined which genes were upregulated,” Strome said.
Analysis of the chromosomes in the offspring’s germline tissue showed that the upregulated genes still lacked the repressive histone mark, while the mark had been restored on the genes that were not upregulated.
“In the germline of the offspring, some genes were aberrantly turned on and stayed in the state lacking the repressive mark, while the rest of the genome regained the mark, and that pattern was passed on to the grandoffspring,” Strome explained. “We speculate that if this pattern of DNA packaging is maintained in the germline, it could potentially be passed on for numerous generations.”
In the grandoffspring, the investigators observed a range of developmental effects, including some worms that were completely sterile. This mix of outcomes is due to how chromosomes get distributed during the cell divisions that produce sperm and eggs, resulting in many different combinations of chromosomes that can be passed on to the next generation.
Researchers in Strome’s lab have been studying epigenetic inheritance in C. elegans for years, and she said this paper represents the culmination of their work in this area. She noted that other scientists researching mammalian cells in culture have reported results very similar to her lab’s findings in worms, although those studies did not show transmission across multiple generations.
“This looks like a conserved feature of gene expression and development in animals, not just a weird worm-specific phenomenon,” she said. “We can do amazing genetic experiments in C. elegans that can’t be done in humans, and the results of our experiments in worms can have broad implications in other organisms.”
Reference: “Sperm-inherited H3K27me3 epialleles are transmitted transgenerationally in cis” Kiyomi Raye Kaneshiro, Thea A. Egelhofer, Andreas Rechtsteiner, Chad Cockrum and Susan Strome, 26 September 2022, Proceedings of the National Academy of Sciences. DOI: 10.1073/pnas.2209471119
The co-first authors of the paper are Kiyomi Kaneshiro, who worked on the study as a graduate student in Strome’s lab and is currently a postdoctoral researcher at the Buck Institute for Research on Aging, and UCSC research associate Thea Egelhofer. The coauthors also include bioinformaticist Andreas Rechtsteiner and graduate student Chad Cockrum (now at IDEXX Laboratories). This work was supported by the National Institutes of Health.
